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. 2018 Oct 25;9:285. doi: 10.1186/s13287-018-1020-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Characterization and flow cytometry analysis of ADSCs. a P3 ADSCs adopted a uniform fibroblast-like, spindle-shaped population. b P6 ADSCs adopted increased aging or dedifferentiated shape with irregularity. Flow cytometry revealed that the level of CD90 and Sca-1 surface antigens of P3 and P6 ADSCs were reduced gradually with statistically significant differences (*P < 0.05; c, d) Scale bar = 100 μm. P3, passage 3; P6, passage 6; ADSC, adipose-derived stem cell