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Gene transfection was adopted to overexpress NTN-1 by ADSCs. a, b Slight difference was observed in the transduction ratio between NTN-1 and GFP in ADSC cells. c–e Western blot analysis, statistical analysis, and PCR analysis verified a significantly high expression of Netrin-1 in N-ADSCs group as compared to no expression in the ADSCs group (P < 0.05). Scale bar = 100 μm. *P < 0.05. ADSC, adipose-derived stem cell; GFP, green fluorescent protein; N-ADSC, Netrin-1-transfected ADSC
