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. 2018 Oct 25;9:284. doi: 10.1186/s13287-018-1028-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Hypoxia significantly increased apoptosis in aged MSCs. a Representative immunofluorescence images of terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) (green fluorescence) and 4,6-diamidino-2-ph under normal conditions and after hypoxia/serum deprivation (H/SD). b Quantification of the rate of apoptosis of BM-MSCs is shown as the percentage of apoptotic cells. c Quantification of apoptosis is shown as the percentage of cells (with marker of annexin in early and late apoptotic stages). Early apoptosis: annexin V+/PI−; late apoptosis: V+/PI+. Data are expressed as the means ± SEM; n = 5; *p < 0.05. d Representative results of the FACS analysis in BM-MSCs under normal conditions and H/SD viable cells: annexin V−/PI−; early apoptosis: annexin V+/PI−; late apoptosis: V+/PI+; necrotic: V−/PI+