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. 2018 Oct 25;9:284. doi: 10.1186/s13287-018-1028-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — IGF-1 knockdown decreased apoptosis. a Representative terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) images of BM-MSCs treated with H/SD with or without IGF-1 siRNA (scale bars, 20 μm). b The quantification result of the apoptotic BM-MSCs with or without IGF-1 siRNA. c Representative results of the FACS analysis of BM-MSCs under normal and H/SD conditions with or without IGF-1 siRNA administration. d Quantification of apoptosis is presented as the percentage of cells with the annexin marker in the early and late apoptotic stages with or without IGF-1 siRNA. Data are expressed as the means ± SEM; n = 5; *p < 0.05