Table 3.
Primer pairs used in PCR identification.
| Primer Name | Primers (5′-3′) | Product (bp) |
|---|---|---|
| YunnanWMV8-2017-1/2/3/4/5-F | GTGAAGAAAGATGAAGGAT | 561 |
| YunnanWMV8-2017-1/2/3/4/5-R | CTATCCAGGGCCTCCCATCA | |
| YunnanDENV2017-1/2/3-F | AGGAGCCCTGTGGGACGTCCC | 1836 |
| YunnanDENV2017-1/2/3-R | TTTTCTTCCACTGGCAAACTCCTT | |
| YunnanDNV2017-1/2/3-F | ACTGGACCAACCGTTGGTG | 387 |
| YunnanDNV2017-1/2/3-R | AGGGCTATGTGCGTTAACAAT | |
| YunnanDNV2017-4/5-F | ACAAAACAAACTCATCAGTCGGC | 429 |
| YunnanDNV2017-4/5-R | TTAGATGATGTAAGGGTTTTGGTTG | |
| YunnanDNV2017-6/7/8-F | CTACTAGCAATGGTTAAACTGG | 801 |
| YunnanDNV2017-6/7/8-R | TCAAGAATCCGGCTGTTTGGTA | |
| YunnanMCCV2017-1/2-F | GCCGGAGCTCTGCAGATAT | 645 |
| YunnanMCCV2017-1/2-R | CCGGAGCTCAGACGTGTGCT | |
| YunnanJEV2017-1/2-F | AGCCGGAGCTCTGCAGATAT | 870 |
| YunnanJEV2017-1/2-R | CTACAGACGTGTGCTCTTC | |
| YunnanJEV2017-3-F | TTTAACTGTCTGGGAATGG | 1064 |
| YunnanJEV2017-3-R | ACCAACCTCCCCACAGGGG | |
| YunnanJEV2017-4/5/6/7-F | ATGAAGCTATCAAACTTTCAAG | 2001 |
| YunnanJEV2017-4/5/6/7-R | GGCATGCACATTGGTCGCT | |
| JEV-F* | CTATTGGTCGCTCCGGCTTACAGT | 1500 |
| JEV-R* | TGTCAATGGCGCAGCCAGTGTC |
*
The primers used in JEV identification after viral isolation.