Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 18;8:358. doi: 10.3389/fcimb.2018.00358

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Gonzalez Rivas, Ximenez, Nieves-Ramirez, Moran Silva, Partida-Rodríguez, Hernandez, Rojas Velázquez, Serrano Vázquez and Magaña Nuñez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

(A) Purification under denaturing conditions of rEhCRT. Proteins were visualized by 12% SDS-PAGE and Coomassie staining; (1) cell lysate with plasmid, (2) cell lysate with plasmid after IPTG induction, (3) molecular weight, (4) rEhCRT eluid from Ni-NTA Agarose column, molecular weight marker are indicated in kilodaltons. (B) Westernblot of rEhCR. The reactivity of the rEhCRT was tested against sera from the different groups studied; (1) 5 μg of rEhCRT electrotransfered to NC membrane, (2–3) serum from control individuals (NEG), (4–6) serum of AP-ALA patients, (7–8) R-ALA patients, and (9–10) reactivity against E. coli-LPS antibody.