Figure 3.

miR-29b is expressed by NKDIs and regulates NK cell development. (A) Freshly isolated tonsillar stage 3 cells were incubated for 24 hours with FICZ or DMSO, and miR-29b expression was determined by quantitative RT-PCR. (B) Stage 3 NKDI cells were cultured ex vivo in the presence of IL-15 and IL-1β and infected with an empty lentiviral vector (empty vector; negative control), cultured with the AHR inhibitor CH223191 (positive control), or infected with the miR-29b lentiviral knockdown (miR-29B KD). In total, 6 independent studies were performed with stage 3 NK cells from 10 primary human donors. (C) A representative figure is depicted. Student t test, *P < .05. Error bars indicate SD.