Table 2.
Flow cytometry antibodies for NKDI isolation and postculture analyses
| Antigen | Clone | Color | Source | Identifier |
|---|---|---|---|---|
| CD3 | BW264/56 | PerCP-Vio700, Viogreen | Miltenyi Biotec | 130-097-582, 130-096-910 |
| CD4 | VIT4 | Viogreen | Miltenyi Biotec | 130-113-221 |
| CD5 | L17F12 | BV510 | Biolegend | 364018 |
| CD14 | TÜK4 | Viogreen | Miltenyi Biotec | 130-096-875 |
| CD20 | LT20 | Viogreen | Miltenyi Biotec | 130-096-904 |
| CD34 | AC136 | APC | Miltenyi Biotec | 130-113-176 |
| CD45 | 2D1 | APC-H7 | BD Biosciences | 560178 |
| CD56 | NCAM16.2 | BV421 | BD Biosciences | 562751 |
| CD94 | REA113 | Biotin | Miltenyi Biotec | 130-098-966 |
| CD117 | REA787 | PE | Miltenyi Biotec | 130-111-592 |
| CD123 | AC145 | Viogreen | Miltenyi Biotec | 130-106-654 |
| CD294 | BM16 | AF647 | BD Biosciences | 558042 |
| FcεR1α | AER-37 | BV510 | Biolegend | 334626 |
| IFN-γ | B27 | AF700 | BD Biosciences | 557995 |
| KIR2D | NKVFS1 | Biotin | Miltenyi Biotec | 130-092-904 |
| KIR3DL1/2 | 5.133 | Biotin | Miltenyi Biotec | 130-095-201 |
| NKG2A | REA110 | Biotin | Miltenyi Biotec | 130-098-819 |
| NKG2C | REA205 | Biotin | Miltenyi Biotec | 130-103-750 |
| NKp44 | 2.29 | VioBright-fluorescein isothiocyanate | Miltenyi Biotec | 130-104-985 |
| NKp80 | REA845 | Biotin | Miltenyi Biotec | 130-112-597 |
APC, allophycocyanin; PE, phycoerythrin.