Table 2. Biosynthesis of PHAs by Aeromonas sp. strains grown in shake flasks during one-stage and two-stage process.

Strain	Nutrient limitation	CDW (g/l)	PHA (wt%)	Monomer composition (mol%)
				3HB	3HV
(1) One-stage process: Cultures were conducted in mineral medium for 48 h
Aeromonas sp. AC_01
Pure glycerol	N	1.69 ± 0.04	7.8 ± 0.09	100	0
	P	1.39 ± 0.01	10.8 ± 0.43	100	0
Crude glycerol	N	2.02 ± 0.11	4.4 ± 0.54	100	0
	P	1.62 ± 0.12	11.1 ± 0.2	100	0
Aeromonas sp. AC_02
Pure glycerol	N	1.48 ± 0.04	5.0 ± 0.13	89.73	10.27
	P	0.95 ± 0.005	20.7 ± 0.95	100	0
Crude glycerol	N	1.41 ± 0.12	6.5 ± 0.63	100	0
	P	1.22 ± 0.01	10.0 ± 0.21	100	0
Aeromonas sp. AC_03
Pure glycerol	N	1.20 ± 0.05	3.6 ± 0.09	100	0
	P	0.70 ± 0.005	16.4 ± 0.45	100	0
Crude glycerol	N	1.27 ± 0.03	9.2 ± 0.49	100	0
	P	1.20 ± 0.02	3.3 ± 0.15	100	0
(2) Two-stage process: Cultures were conducted in LB medium for 8 h and then with glycerol for 40 h
Aeromonas sp. AC_01
Pure glycerol	N	2.70 ± 0.12	3.4 ± 0.11	100	0
	P	1.70 ± 0.004	3.0 ± 0.14	100	0
Crude glycerol	N	2.72 ± 0.04	3.6 ± 0.18	100	0
	P	2.33 ± 0.03	3.8 ± 0.14	100	0
Aeromonas sp. AC_02
Pure glycerol	N	2.88 ± 0.05	3.7 ± 0.16	100	0
	P	3.00 ± 0.05	10.5 ± 0.05	100	0
Crude glycerol	N	2.00 ± 0.10	2.8 ± 0.19	100	0
	P	2.65 ± 0.05	13.6 ± 0.53	100	0
Aeromonas sp. AC_03
Pure glycerol	N	2.53 ± 0.08	4.7 ± 0.44	100	0
	P	2.32 ± 0.05	42.0 ± 0.57	100	0
Crude glycerol	N	2.58 ± 0.13	5.6 ± 0.40	100	0
	P	3.06 ± 0.09	22.1 ± 0.30	100	0

Notes:

Data were expressed as average value and standard deviation of three parallel samples.

3HB, 3-hydroxybutyrate; 3HV, 3-hydroxyvalerate.