Figure 3.

UCA1 serves as a ceRNA and negatively regulates miR-204 expression in PTC.

Notes: (A) Schematic of the construction of Wt- or Mut-UCA1 3′-UTR vectors is indicated. (B) Relative luciferase activities were analyzed in TPC-1 cells cotransfected with the Wt-UCA1 or Mut-UCA1 reporter plasmid and miR-204 mimic or miR-NC. (C) miR-204 expression level was determined in TPC-1 cells transfected with si-UCA1 or si-NC. U6 was used as an internal control. (D) UCA1 expression level was determined in TPC-1 cells transfected with miR-204 mimic, miR-NC, miR-204 inhibitor (anti-miR-204), or anti-miR-NC. GAPDH was used as an internal control. (E) miR-204 expression level was determined in TPC-1 cells transfected with si-NC or si-UCA1 and with/without anti-miR-204. U6 was used as an internal control. (F–H) Cell proliferation, migration, and invasion were determined in TPC-1 cells transfected with si-NC or si-UCA1 and with/without anti-miR-204. All data are representative of three independent experiments and expressed as mean±SD. *P<0.05 and **P<0.01.

Abbreviations: ceRNA, competing endogenous RNA; PTC, papillary thyroid carcinoma; Wt, wild type; Mut, mutant type; NC, negative control.