Figure 4.

Glucose concentration, cell proliferation, and IFN-γ production in T cells after co-culture with 5637 and T24 cells for 5 days.

Notes: (A) Glucose concentration in culture medium was detected using a Glucose Assay Kit. (B) T cell proliferation was investigated by CCK-8. (C) IFN-γ production of T cells was investigated by ELISA. (D) Morphology of T cells co-cultured with 5637 and T24 cells directly for 24 hours was observed under a microscope and compared with T cells cultured alone (100×). **P<0.01; ns, not significant.

Abbreviations: CCK-8, Cell Counting Kit-8; IFN-γ, interferon-γ.