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. 2018 Oct 8;53(6):2409–2422. doi: 10.3892/ijo.2018.4586

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Mst1 affects A549 cell migration and proliferation. (A-C) Expression levels of proliferation-associated proteins were analyzed by western blotting in A549 cells following Ad-Mst1 transduction. (D and E) Cell proliferation was measured via EdU staining. The number of EdU-positive cells was recorded. (F) A549 cells transduced with Ad-Mst1 were isolated using 0.25% trypsin and cell viability was determined using a LDH release assay. (G and H) Isolated cells were seeded onto Transwell units and a Transwell assay was performed to evaluate cell migration; the number of migrated cells was recorded. (I-K) Chemokines CXCR4 and CXCR7 were measured via western blotting in response to Ad-Mst1 transduction. ^*P<0.05. Ad, adenovirus; CXCR, C-X-C chemokine receptor; EdU, 5-ethynyl-2′-deoxyuridine; LDH, lactate dehydrogenase; Mst1, mammalian STE20-like kinase 1.