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. 2018 Oct 8;53(6):2409–2422. doi: 10.3892/ijo.2018.4586

Figure 4.

Figure 4

Mst1 overexpression suppresses mitochondrial respiratory function. (A) ATP production was measured in A549 cells following Ad-Mst1 transduction. (B-D) Mitochondrial respiratory complex protein activity was evaluated via ELISA analyses. Ad-Mst1 transduction decreased the activity of mitochondrial respiratory complex proteins in A549 cells. (E-H) Western blotting was used to analyze the protein expression levels of mitochondrial respiratory complexes. GAPDH was used as a loading control for western blotting. (I and J) Glucose uptake and lactate production were measured in the medium following Ad-Mst1 transduction. *P<0.05. Ad, adenovirus; CII-30, complex II; CIII-core2, complex III subunit core 2; CIV-II, complex IV subunit II; Mst1, mammalian STE20-like kinase 1.