Figure 5.

ROCK1/F-actin pathways are regulated by Mst1 and contribute to A549 cell apoptosis. (A-C) Western blotting was performed to analyze the protein expression levels of ROCK1 and F-actin. (D and E) Immunofluorescence of F-actin in response to treatment with Y-27632, which is an inhibitor of the ROCK1 pathway that was used to inhibit ROCK1 activation. Suppression of ROCK1 attenuated F-actin expression. (F and G) Staining of TUNEL-positive cells and F-actin. More TUNEL-positive cells were associated with decreased F-actin expression. (H) LDH release assay was used to evaluate the pro-apoptotic effects of Mst1. ^*P<0.05. Ad, adenovirus; LDH, lactate dehydrogenase; Mst1, mammalian STE20-like kinase 1; ROCK1, Rho-associated coiled-coil containing protein kinase 1; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling.