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. 2018 Oct 8;53(6):2409–2422. doi: 10.3892/ijo.2018.4586

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Mst1-Rho-associated coiled-coil containing protein kinase 1-F-actin pathways control A549 cell migration and proliferation. (A and B) Immunofluorescence analysis of F-actin. The average length of filopodia was measured in response to Ad-Mst1 transduction and Y-27632 treatment. (C and D) Transwell assay was conducted on A549 cells following Ad-Mst1 transduction and Y-27632 administration. (E and F) EdU assay was used to observe cellular proliferation. The number of EdU-positive cell was recorded. (G-I) Immunofluorescence analysis of cyclin proteins. Cyclin E and cyclin D1 were observed and the fluorescence intensity was measured in response to Ad-Mst1 transduction and Y-27632 treatment. ^*P<0.05. Ad, adenovirus; EdU, 5-ethynyl-2′-deoxyuridine; Mst1, mammalian STE20-like kinase 1.

Mst1-Rho-associated coiled-coil containing protein kinase 1-F-actin pathways control A549 cell migration and proliferation. (A and B) Immunofluorescence analysis of F-actin. The average length of filopodia was measured in response to Ad-Mst1 transduction and Y-27632 treatment. (C and D) Transwell assay was conducted on A549 cells following Ad-Mst1 transduction and Y-27632 administration. (E and F) EdU assay was used to observe cellular proliferation. The number of EdU-positive cell was recorded. (G-I) Immunofluorescence analysis of cyclin proteins. Cyclin E and cyclin D1 were observed and the fluorescence intensity was measured in response to Ad-Mst1 transduction and Y-27632 treatment. ^*P<0.05. Ad, adenovirus; EdU, 5-ethynyl-2′-deoxyuridine; Mst1, mammalian STE20-like kinase 1.