Table I.
Genes evaluated by RT-qPCR, according to GenBank access, primer sequence, melting temperature and product size.
| Gene | GenBank | Primers 5′→3′ | Temperature (°C) | Product size (bp) |
|---|---|---|---|---|
| POU5F1 | NM_001173531.1 | F: acttcactgcactgtactcctc | 62 | 159 |
| R: aggttctctttccctagctcctc | ||||
| NANOG | NM_024865.2 | F: catcctgaacctcagctacaaaca | 62 | 99 |
| R: ttgctattcttcggccagttgt | ||||
| CD133 | NM_001145848 | F: ggcccagtacaacactacc | 62 | 71 |
| R: cgcctcctagcactgaatt | ||||
| CD44 | NM_000610.3 | F: caaccgttggaaacataacc | 60 | 226 |
| R: caagtgggaactggaacgat | ||||
| BMI1 | NM_005180.8 | F: gctgccaatggctctaatgaa | 60 | 189 |
| R: tgctgggcatcgtaagtatctt | ||||
| SNAI1 | NM_003068.4 | F: caaggaatacctcagcctgg | ||
| R: catctgagtgggtctggagg | 60 | 218 | ||
| S100A9 | NM_002965.3 | F: aaagagctggtgcgaaaaga | ||
| R: gtgtccaggtcctccatgat | 60 | 92 | ||
| IVL | NM_005547.3 | F: caagacattcaaccagccct | ||
| R: tagcggacccgaaataagtg | 60 | 185 | ||
| GAPDH | NM_001256799 | F: gcatcctgggctacactga | ||
| R: ccaccaccctgttgctgta | 60 | 162 |