Figure 3.

Knockdown of CPNE1 decelerates the cell cycle in NSCLC cells. (A and B) Flow cytometric analysis of A549 and H1299 cells. Cells were harvested and stained with propidium iodide. The percentage of cells in each cell cycle phase is presented in the inset of each panel, in which the values represent the mean ± standard deviation of three measurements. (C) Signaling molecules were detected, and the data demonstrated that p-AKT, p-ERK, cyclin-A1, cyclin-B1 and cyclin-E1 levels were markedly decreased in sh-CPNE1 compared with those in sh-NC. ^*P<0.05 and ^**P<0.01 vs. sh-NC. CPNE1, Copine 1; NSCLC, non-small cell lung cancer; p, phosphorylated; AKT, protein kinase B; ERK, extracellular signal regulated kinase; sh-NC, negative control cells; sh-CPNE1, CPNE1-silenced cells.