Figure 4.

Silencing of CPNE1 inhibits the migratory and invasive abilities of NSCLC cells, and its associated pathways. (A and B) CPNE1 silencing inhibits invasion and migration of NSCLC cells. Assays were performed in a Transwell plate. The cells that had migrated were stained with crystal violet and counted in at least three microscopic fields (magnification, ×100). Subsequently, the cells were treated as above and allowed to invade through the Matrigel-coated membrane in Transwell plates. Invasive cells were stained with crystal violet and counted under a light microscope (magnification, ×100). (C and D) Snail, MMP2, MMP9 were markedly decreased in sh-CPNE1 compared with sh-NC. ^*P<0.05 and ^***P<0.001 vs. sh-NC. CPNE1, Copine 1; NSCLC, non-small cell lung cancer; MMP, matrix metalloproteinase; sh-NC, negative control cells; sh-CPNE1, CPNE1-silenced cells.