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. 2018 Oct 1;53(6):2397–2408. doi: 10.3892/ijo.2018.4579

Figure 1.

Figure 1

Induction of downregulated CD147 expression in melanoma A375 cells. (A) Transfection efficiency of pLK0.1-sh-CD147 (C1 or C2) and the control sh-Mock vectors in melanoma A375 cells was determined via western blotting to confirm CD147 protein expression. Transfection with the sh-CD147-C2 vector notably inhibited CD147 protein expression in A375 cells. (B) Stable downregulation of CD147 in A375 sh-CD147-C1 and -C2, and the control sh-Mock-transfected cells was assessed via flow cytometry analysis. Transfection with pLK0.1-sh-Mock vector into A375 is referred to as Sh-Mock. C1 or C2, transfection with pLK0.1-sh-CD147 vector; CD147, cluster of differentiation 147; sh, short hairpin RNA.