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. 2018 Oct 1;53(6):2397–2408. doi: 10.3892/ijo.2018.4579

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Inhibition of CD147 promotes the apoptosis of melanoma cell lines. (A) Representative dot plots of Annexin V-FITC and PI staining. After A375 cells were stably transfected with sh-Mock (left) or sh-CD147 vector (right), apoptosis analysis was performed by flow cytometry. (B) Apoptotic rate of sh-CD147- and sh-Mock-transfected A375 cells. The percentage of apoptotic cells was significantly higher with decreased CD147 expression. (C and D) Protein expression levels of apoptosis-associated cle-PARP in sh-CD147- and sh-Mock-transfected A375 and SK-28 cells. (E) Transmission electron microscopy images of apoptosis of sh-CD147-transfected and sh-Mock-transfected A375 cells. Apoptotic bodies are indicated by arrows. n=3 for each experiment. Scale bar=2 μm. ^**P<0.01. CD147, cluster of differentiation 147; cle-PARP, cleaved poly (ADP-ribose) polymerase; FITC, fluorescein isothiocyanate; PI, propidium iodide; sh, short hairpin RNA.