Figure 6.

Transwell invasion assay was performed with U251MG and U87MG cells with or without COL1A1 siRNA treatment. (A and C) Representative micrographs (magnification, ×100) of invaded cells in Transwell assays (plus Matrigel). Astrocytoma or glioblastoma cells (1×10⁴) were seeded as indicated and co-cultured with COL1A1 siRNA for 24 h. (B and D) Graphic representation of the quantification of invaded cells. All data are presented as the means ± standard deviation of three independent experiments (scale bar, 100 μm). Data in (A and B) are from the U251MG cells, while those in (C and D) are from the U87MG cells. ^**P<0.01 and ^***P<0.001. COL1A1, collagen type I α1.