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. 2018 Sep 18;53(6):2379–2396. doi: 10.3892/ijo.2018.4565

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Copyright: © Turano et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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LiCl inhibits cell plasticity. Molecular characterisation of survived cells. (A) Brightfield images (×10 magnification) of cells disaggregated from T88 and T93 spheroids obtained in medium containing (LiCl) or not containing (untreated) LiCl. (B) Confocal images of cells dual-stained with anti-N-cadherin (red)/anti-E-cadherin (green) and anti-CD133 (red)/anti-CD44v6 (green) antibodies Nuclei were counterstained with DAPI (blue). Magnification, ×20. (C) Real-time quantification of Snail and Twist1 mRNA expression in T88 and T93 adherent cells before tumoursphere formation (1st step), cells disaggregated from untreated tumourspheres (drop cells) and cells disaggregated from treated tumourspheres (drop cells in LiCl). This experiment was performed two times with similar results. A typical experiment is shown. Numbers on the top of each of the bars represent the precise value of relative expression for each sample, as calculated by the ΔΔCq method.