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. 2018 Sep 18;53(6):2379–2396. doi: 10.3892/ijo.2018.4565

Figure 12.

Figure 12

Expression of Nanog, ALDH1, Snail, LGR5, Oct4 and Sox-2 in cells disaggregated from T88 and T93 spheroids. Confocal images of cells disaggregated from T88 and T93 spheroids obtained in medium containing (LiCl) or not containing (untreated) LiCl. Cells were dual-stained with anti-Nanog (red)/anti-ALDH1 (green), anti-Snail (red)/anti-LGR5 (green) and anti-Oct4 (red)/anti-Sox-2 (green) antibodies. Nuclei were counterstained with DAPI (blue).