Table I.
Antibodies and dilutions used for immunofluorescence staining.
Antibody: Dilution (Cat. no., provider) | Antibody: Dilution (Cat. no., provider) |
---|---|
Pan-cytokeratin (CK): 1:50 (MA5-13203, Invitrogen, Thermo Fisher Scientific) | E-cadherin: 1:50 (ab76055, Abcam) |
Nanog: 1:50 (3580, Cell Signaling Technology) | N-cadherin: 1:50 (ab76057, Abcam) |
Sox2: 1:50 (4900, Cell Signaling Technology) | Snail: 1:150 (ab180714, Abcam) |
Oct4: 1:50 (2840, Cell Signaling Technology) | LGR5: 1:50 (sc135238, Santa Cruz Biotechnology) |
Vimentin: 1:200 (5741, Cell Signaling Technology) | ALDH1: 1:50 (ab24343, Abcam) |
CD44: 1:500 (5640, Cell Signaling Technology) | CD133: 1:50 (ab16518, Abcam) |
β-catenin: 1:100 (9581, Cell Signaling Technology) | CD44v6: 1:50 (BBA13, R&D Systems) |
Sox2, sex determining region Y-box 2; Oct4, octamer-binding transcription factor 4; LGR5, leucine-rich repeat-containing G-protein coupled receptor 5; ALDH1, aldehyde dehydrogenase 1.