Figure 11.

Effect of a p38 inhibitor on the G2/M phase arrest induced by Areno and Helle in U-87 cells. Following treatment with 20 ng/ml of (A) Areno and (B) Helle, in the presence or absence of 1 μM SB203580, a specific inhibitor of p38 MAPK and its negative control SB202474 for 48 h, cell cycle analysis was performed using a FACSCanto flow cytometer as described in Materials and methods. Data are presented as the mean ± standard deviation from three independent experiments. ^§P<0.001 and ^†P<0.0001 vs. respective control. Areno, arenobufagin; Helle, hellebrigenin.