Table 1. Optical Parameters of the SLBs Obtained with Fresnel-Layer Modeling of the Reflectance Spectra from SPR Measurements, Averaged over Three Individual Measurements with Standard Errors of the Mean (See the Supporting Information for Calculation of SEMs).
| lipid | d (nm) | n1 | n2 | dn/dλ (10–3 nm–1) |
|---|---|---|---|---|
| Method 3:Algorithm-BasedDual-WavelengthModeling | ||||
| DOPC | 4.90 ± 0.06 | 1.4776 ± 0.0013 | 1.4728 ± 0.0013 | –0.0420 ± 0.0006 |
| DOPC–DOPS | 4.89 ± 0.10 | 1.4827 ± 0.0018 | 1.4778 ± 0.0018 | –0.0427 ± 0.0007 |
| POPC | 4.96 ± 0.03 | 1.4776 ± 0.0017 | 1.4728 ± 0.0017 | –0.0423 ± 0.0003 |
| DOPC–Sm–Chol | 6.04 ± 0.33 | 1.4889 ± 0.0069 | 1.4835 ± 0.0069 | –0.0463 ± 0.0009 |
| DPPC | 76.13 ± 3.34 | 1.3614 ± 0.0016 | 1.3567 ± 0.0014 | –0.0410 ± 0.0020 |
| Method 4: InverseDual-WavelengthModeling | ||||
| DOPC | 4.70 ± 0.16 | 1.4845 ± 0.0025 | 1.4797 ± 0.0025 | –0.0421 ± 0.0001 |
| DOPC–DOPS | 5.04 ± 0.15 | 1.4781 ± 0.0062 | 1.4732 ± 0.0061 | –0.0428 ± 0.0009 |
| POPC | 4.96 ± 0.04 | 1.4774 ± 0.0016 | 1.4725 ± 0.0016 | –0.0421 ± 0.0001 |
| DOPC–Sm–Chol | 6.13 ± 0.06 | 1.4855 ± 0.0033 | 1.4800 ± 0.0032 | –0.0459 ± 0.0003 |
| DPPCa | 37.36 ± 1.26 | 1.3829 ± 0.0019 | 1.3792 ± 0.0016 | –0.0320 ± 0.0031 |
a
The average thickness of the DPPC vesicle layer was first calculated from Sauerbrey analysis of the QCM-Z data by using an adlayer mass density of c = 1.05 g/mL. Subsequently, the refractive indices were calculated by Fresnel-layer modeling of SPR measurements, with the thickness value derived from the QCM-Z measurements used as a fixed value in the Fresnel modeling. All other values in the table were determined from modeling of the SPR reflectance spectra.