Fig. 4.

UCHL1 promotes RA-induced neurite outgrowth of NB cells. a-b SH-SY5Y and SK-N-BE (2) cells were treated with DMSO or RA (10 μM) for 3, 5 and 7 days, protein and mRNA were collected. UCHL1 expression was determined at the protein and mRNA levels by immunoblotting analysis and quantitative real-time PCR. c SH-SY5Y and SK-N-BE (2) cells were infected with control lentivirus (shNC) or lentivirus expressing shRNA targeting UCHL1 (shUCHL1). UCHL1 expression was measured by immunoblotting analysis. d shNC and shUCHL1 SH-SY5Y cells were treated with DMSO or RA (10 μM) for 3, 5 and 7 days. The morphology was examined. Scale bars, 50 μm. e SH-SY5Y cells were treated with DMSO or RA (10 μM) for 3, 5 and 7 days in the presence of LDN57444 (10 μM) or not. The morphology was examined. Scale bars, 50 μm. f shNC and shUCHL1 SK-N-BE (2) cells were treated with DMSO or RA (10 μM) for 7 days. The morphology was examined. Scale bars, 50 μm. Values are shown as mean ± S.E.M. and statistical significance indicated as * P < 0.05