Fig. 7.

UCHL1 promotes RA-induced proliferation inhibition of NB cells. shNC and shUCHL1 SH-SY5Y cells were treated with DMSO or RA (10 μM) for 3, 5 or 7 days. a BrdU assay showed the proliferation of shNC and shUCHL1 SH-SY5Y cells after DMSO or RA treatment for 3 days, and the percentage of BrdU⁺ SH-SY5Y cells was analyzed by flow cytometry. b CCK8 assay showed the proliferation of shNC and shUCHL1 SH-SY5Y cells at the indicated times. c The protein expression of p21, cyclin D1 and UCHL1 were measured by immunoblotting analysis at the indicated times, and the densitometry of the bands was quantified using ImageJ software. shNC and shUCHL1 SK-N-BE (2) cells were treated with DMSO or RA (10 μM) for 7 days. d CCK8 assay showed the proliferation of shNC and shUCHL1 SK-N-BE (2) cells. e The protein expression of p21, cyclin D1 and UCHL1 were measured by immunoblotting analysis, and the densitometry of the bands was quantified using ImageJ software. Values are shown as mean ± S.E.M. and statistical significance indicated as * P < 0.05