Table 2.

Primers and experimental conditions used for qPCR analysis

Gene	Tm (°C)	Primer concentration (nM)	Sequence	PCR product size (bp)
18S	60	300	5′- ATCGGGGATTGCAATTATTC -3′	130
			3′- CTCACTAAACCATCCAATCG -5′
GAPDH	60	300	5′- ACAGTTGCCATGTAGACC -3′	95
			3′- TTTTTGGTTGAGCACAGG -5′
COX2	60	400	5′- GGAGAAAAGGAAATGTCTGC -3′	186
			3′- GTAGGCAGGAGAACATATAAC -5′
RUNX2	60	400	5′- GCAGTATTTACAACAGAGGG -3′	112
			3′- TCCCAAAAGAAGTTTTGCTG -5′
OPN	60	400	5′- GACCAAGGAAAACTCACTAC -3′	84
			3′- CTGTTTAACTGGTATGGCAC -5′