Table 10.
miRNA studies in other kidney diseases
| PMID | Author | Year | Sample | Patients | Ethnics | Methodology | Main findings |
|---|---|---|---|---|---|---|---|
| 28667184 | Cárdenas-González | 2017 | Urine | Discovery cohort: 10 LN 10 HC Confirmation stage: 89 LN 119 HC Replication stage: 86 LN 37 SLE 30 HC |
Caucasian | qRT-PCR miRNA in situ hybridisation 2402 urinary microRNAs |
Downregulation of miR-3201 and miR-1273e (> threefold; p < 0.0001) in LN patients Association of miR-3201 and miR-1273e expression with endocapillary glomerular inflammation (p < 0.01) |
| 28380212 | Domenico | 2017 | Urine | 41 graft dysfunction 8 stable patients |
Brazil | qRT-PCR: miR-142-3p | Overexpression of urinary miR-142-3p in the acute tubular necrosis group (p < 0.001) and acute rejection group (p < 0.005) in comparison with stable patients Trend to higher urinary miR-142-3p expression, in the acute tubular necrosis group, compared with the acute rejection group (p = 0.079) ROC analysis: AUC was 0.77 (95%CI 0.62–0.92; p = 0.010). The cut-off selected was 2.03 and the resulting parameters were sensitivity = 92%; specificity = 87%; positive predictive value = 90% and a negative predictive value = 87% (p < 0.001) |
| Blood | Overexpression of urinary miR-142-3p when acute tubular necrosis was compared with stable patients (p < 0.05) and with the acute rejection group (p < 0.05) No difference was found in the comparison between the stable patients and acute rejection groups (p = 0.921) ROC analysis: AUC was 0.75 (95%CI 0.56–0.94; p = 0.016). The cut-off selected was 1.59 and the resulting parameters were sensitivity = 75%; specificity = 63%; positive predictive value = 81%, and a negative predictive value = 50% (p < 0.05) |
||||||
| 24489795 | Ben-Dov | 2014 | Urine | 20 CKD 20 ADPKD |
barcoded adapter ligation, Illumina Genome Analyzer II |
mir-143(2) members were 2.9-fold higher in urine cells from ADPKD compared to other CKD patients Expression levels of mir-133b(2) (4.9-fold) and mir-1(4) (4.4-fold) were lower in ADPKD |
|
| 25197634 | Hu Yang-Yang | 2014 | Serum | 60 Nephrolithiasis, 50 HC |
Asian | qRT-PCR: miR-155 |
The median levels of serum and urinary levels of miR-155 were significantly higher in nephrolithiasis patients than in controls eGFR inversely correlated with urinary level of miR-155 |
| 22685016 | Guan | 2012 | Urine | 12 SLE 10 NecGN |
qRT-PCR | The degree of proteinuria significantly correlated with the urinary sediment level of miR-339-3P (r = 0.636; p < 0.001), but not other miRNA targets Urinary sediment miR-221 level correlated with serum C3 level (r = 0.658; p = 0.02). In the SLE group, urinary sediment miRNA levels did not correlate with the histological activity or chronicity index of the renal biopsy specimen |
|
| 21987229 | Wang | 2012 | Urine | 40 SLE 13 HC |
Asian | qRT-PCR: miR-146a and miR-155 | Overexpression of urine miR-146a (2.27 [1.20–5.52] versus 0.87 [0.54–1.18]; p = 0.001) and miR-155 (2.28 [1.16–5.13] versus 0.99 [0.84–1.67]; p = 0.001) in SLE Correlation of eGFR with the level of urinary miR-146a (r = 0.242; p = 0.008), but not miR-155 (r = 0.069; p = 0.456) |
| 20952466 | Wang | 2010 | 40 SLE 30 HC |
Asian | qRT-PCR: miR-146a and miR-155 | Downregulation of serum miR-146a and miR-155 levels, and upregulation of urinary level of miR-146a in SLE eGFR correlated with both serum miR-146a (r = 0.519; p = 0.001) and miR-155 (r = 0.384; p = 0.014) Serum miR-146a inversely correlated with proteinuria (r = − 0.341; p = 0.031) and the SLE Disease Activity Index (r = − 0.465; p = 0.003) |
ADPKD: autosomal dominant polycystic kidney disease; C3: complement component 3; CKD: chronic kidney disease; eGFR: estimated glomerular filtration rate; HC: healthy controls; NecGN: pauci-immune necrotizing glomerulonephritis; LN: lupus nephritis; qRT-PCR: quantitative real-time polymerase chain reaction; RNA: ribonucleic acid; ROC: receiver operating characteristic; SLE: systemic lupus erythematous