Table 8.
miRNA studies in diabetic kidney disease
| PMID | Author | Year | Sample | Patients | Ethnics | Methodology | Main findings |
|---|---|---|---|---|---|---|---|
| 28667184 | Cardenas-Gonzalez | 2017 | Urine Blood | Discovery cohort: 10 DKD, 10 HC Confirmation stage: 58 DKD, 119 HC Replication stage: 74 DKD, 71 DM, 30 HC |
Caucasian | 2402 microRNAs | Downregulation of miR-2861, miR-1915-3p and miR-4532 in patients with diabetic nephropathy, were (> 10-fold; p < 0.0001) Downregulation of miR-2861, miR-1915-3p and miR-4532 associated with eGFR (p < 0.01) and interstitial fibrosis/tubular atrophy (p < 0.05) |
| 28923913 | Liu | 2017 | Blood | T1DM/T2DM DKD/HC |
RNA-Seq | Downregulation of serum miR-25 in patients with diabetes, both with and without nephropathy (p < 0.001) | |
| 26239688 | Argyropoulos | 2015 | Urine | 17 T1DM + mAlb 10 T1DM | qRT-PCR, Exiqon miRNA, qPCR panels 1 and 2 | The development of microalbuminuria was associated with 18 differentially expressed microRNAs The predicted targets of these microRNAs map to biological pathways involved in the pathogenesis and progression of diabetic renal disease. A microRNA signature (miR-105-3p, miR-1972, miR-28-3p, miR-30b-3p, miR-363-3p, miR-424-5p, miR-486-5p, miR-495, miR-548o-3p and for women miR-192-5p, miR-720) achieved high internal validity for microalbuminuria |
|
| 23358711 | Argyropoulos | 2013 | Urine | 10 DM-DKD 10 DM 10 DM + IMA 10 DM + PMA 10 HC |
qRT-PCR miRNA panel |
miRNA-221-3p expression decreased similarly to the comparison of follow up and baseline microalbuminuria samples miR-589 and miR-323b-5p showed a trend to be increased in the urine of patients with overt nephropathy Downregulation of miR-323b-5pand upregulation of: miR-122-5p, miR-429 in PMA patients Appearance of micro-albuminuria was associated with decreased levels of miR-323b-5p and increased urine concentration of miR-429 |
|
| 24223694 | Barutta | 2013 | Urine Exosomes | 12 T1DM + mAlb 12 T1DM + nAlb 10 non-diabetics |
Human TaqMan miRNA Array A | Differential expression of miR-130a, miR-424, miR-155 and miR-145 in urinary exosomes from microalbuminuric T1DM Downregulation of miR-155 and miR-424 and upregulation of miR-130a and miR-145 in micro- compared to normoalbuminuric patients miR-155 and miR-424 could not discriminate between normoalbuminuric diabetic patients and non-diabetic controls Trend to overexpression of miR-145 and miR-130 in normoalbuminuric diabetic patients compared to controls |
|
| 23797704 | Fiorentino | 2013 | Tissue | 8 DM + DKD 4 HC |
qRT-PCR TaqMan miRNA reverse transcription kit |
Overexpression of miR-21 in kidney biopsies from diabetic patients compared to HC Downregulation of miR-217 in diabetic subjects compared to HC |
|
| 20056746 | Krupa | 2010 | Biopsies | 22 DKD | Caucasian | qRT-PCR TaqMan Low Density Array Human MicroRNA Panel 1.0 | Two miRNAs differed by more than two-fold between progressors and nonprogressors, and 12 miRNAs differed between late presenters and other biopsies Late presenters showed the greatest decrease in miR-192 expression Downregulation of miR-192 correlated with tubulointerstitial fibrosis and low eGFR |
DKD: diabetic kidney disease; DM: diabetes mellitus; eGFR:estimated glomerular filtration rate; HC: healthy controls; IMA: intermittent microalbuminuria; mAlb: microalbuminuria; MAlb: macroalbuminuria; nAlb: normoalbuminuria; PMA: persistent microalbuminuria; qRT-PCR: quantitative real-time polymerase chain reaction; RNA: ribonucleic acid; T1DM: type 1 diabetes mellitus; T2DM: type 2 diabetes mellitus