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. 2018 Oct 26;13(10):e0205071. doi: 10.1371/journal.pone.0205071

Fig 4. In vitro IgG antibody secreting cell responses in splenocytes day 5 post challenge.

Fig 4

The cells from the spleens were cultured for 1 day or 5 days and RSV specific IgG antibodies secreted into culture supernatants determined by ELISA. (A) IgG, (B) IgG1, (C) IgG2a and (D) Ratios of IgG2a/IgG1 isotypes levels were analyzed by ELISA using FI-RSV as a coating antigen. Mouse groups are the same as described in Figs 1 and 3. Results are presented as mean (n = 5) ± SEM and were obtained from duplicate experiments. Statistical significances were performed by one-way ANOVA and Tukey’s multiple-comparison tests in GraphPad Prism; *** p<0.001, *p<0.05.