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. 2018 Oct 25;9:278. doi: 10.1186/s13287-018-1033-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Immunologic properties of mESC-FBs. a Expression of immunomodulators induced by inflammatory cytokines. Cells were treated for 24 h with indicated cytokines, alone or in combination. The expression levels of IL6, TNFα and ICAM1 were determined by RT-qPCR analysis. b, c Analysis of IFNγ- and TNFα-induced iNOS and COX2 expression by RT-qPCR (b) and flow cytometry (c). d Comparative analysis of MHC expression in BM-MSCs, mESCs and mESC-FBs by RT-qPCR. Values in RT-PCR analysis are mean ± SD of data from three independent experiments. *p < 0.05, a, b, compared with respective controls (Con); d compared with ESC. The results in flow cytometry are representative experiments performed three times with similar results