. Author manuscript; available in PMC: 2018 Oct 26.

Published in final edited form as: J Autoimmun. 2016 May 6;75:30–38. doi: 10.1016/j.jaut.2016.04.008

Table 5.

Effect of HCQ on antibody complement C1q fixation and C3 activation capacity. Values of C1q fixation and C3b/iC3b/C3 generation are expressed as O.D. at wavelength (λ) = 450. Complement fixing mouse monoclonal aPL antibody FB1 and control non fixing FD1 antibody were used in the ELISA. N=5-7 / experimental group.* different from control antibody FD1, p<0.05; # different from complement fixing antibody FB1, p<0.05.

	OD λ450 nm x 100		C3adesArg (ng/ml)
	C1q binding	C3b/iC3b/C3c	C3adesArg (ng/ml)
FD1 (control)	65±08	17±5	97±23
FB1 (complement activating antibody)	568±47*#	102±12*#	892±54*#
FB1+ HCQ20	392±67*#	81±9*	556±28*#
FB1+HCQ50	197±45*#	38±11*#	235±45*#
FB1+HCQ100	57±12#	15±8#	86±19#
FB1+serum from HCQ-treated mouse	48±14#	21±6#	78±21