Fig. 2.

Melanopsin expression in the cornea revealed by the melanopsin-specific antibody, immunostaining of the mouse cornea with the antimelanopsin antibody made in the lab (see Materials and methods). Images obtained in corneal epithelium (A, B) and near the endothelium (C, D). Panels include comparisons of immunoreactivity in melanopsin wildtype mice (A, C) and knockout mice (B, D). Melanopsin staining in WT mice demonstrated immunoreactive fibers in the epithelial part of the cornea, and fibers and round structures, putative dendritic or endothelial cells, near the endothelial surface. No immunoreactive fibers or cells were detected in the melanopsin knockout mice. Similar findings were obtained in at least 4 different WT and melanopsin knockout mice.