Fig 1. VNUT and/or TRPV4 mRNA expression in human cell lines and mouse tissues.
(A) VNUT, TRPV4 (V4) and GAPDH (GAP) mRNA was detected in human gastric (GES-1) and colonic (CCD 841) epithelial cell lines. (B) Quantitative RT- PCR (qRT- PCR) indicated significantly lower mRNA expression in VNUT-knockout (KO) tissues (Eso; esophagus, Sto; stomach, Int; intestine, Colo; colon) compared to wild-type (WT) tissues (# P < 0.05 vs WT).