Fig 4. TRPV4-mediated current responses in mouse primary colonic epithelial cells.

(A) TRPV4-mediated current responses in mouse primary colonic epithelial cells (mean ± SEM). GSK101 (300 nM) evoked inward current responses in WT primary colonic epithelial cells. Currents in response to ramp-pulses at points a, b and c (left) are shown (middle), with a strong outwardly rectifying current-voltage relationship (reversal potential of 0.4 mV). (B) Summary data of rectification between basal condition (at “a” in Fig 4A) and GSK101-induced currents (at “b” in Fig 4A) in WT cells (n = 5; p < 0.05 vs. Basal). (C) Significantly larger inward current densities (pA/pF) at -60 mV were obtained from WT cells (n = 5) than from TRPV4-KO cells (n = 5; # p < 0.05 vs. WT).