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. 2018 Oct 26;13(10):e0206276. doi: 10.1371/journal.pone.0206276

Fig 5. TRPV4 agonists-induced VNUT-mediated ATP release in CCD 841 and GES-1 cells.

Fig 5

(A) Dose-response ATP release rate curve in CCD 841 cells responding to GSK101 determined EC50 value of 87.5 nM. (B)Dose-response ATP release rate inhibition curve with a TRPV4 specific inhibitor, RN1734 in CCD 841 cells responding to GSK101 (3 μM) determined IC50 value of 142.2 pM. (C, D) GSK101 (GSK, 300 nM)-induced ATP release in GES-1 and CCD 841　cells was significantly inhibited by pre-treatment with the specific VNUT inhibitor clodronate (Clod, 1 μM; p < 0.05). 5,6-EET (1.5 μM)-induced ATP release in GES-1 and CCD 841 cells was also significantly inhibited by pre-treatment with clodronate liposomes (Clod-Lipo, 1 μM; p < 0.05).