Fig 2. The H3.3 chaperone HIRA binds to endogenous CENP-A.
A.) Western blot analysis of IPs using either CENP-A IP (ACA antibody) or Mock IP from soluble pool obtained from SW480 cells that were treated for 72 hours with either control or HJURP siRNA as indicated. ControlMock IP was performed on control siRNA treated cells with no antibody while ControlCpA Ip was performed on control siRNA treated cells with the antibody. B.) Graph indicating the levels of each indicated chaperone in the CENP-A IP shown in A. Levels for each chaperone were normalized to control and also the amount of CENP-A pulled down in either control or HJURP siRNA experiments. Results are obtained from triplicate experiments. C.) Western blot results from IP of soluble GFP or GFP-HIRA.