Table 2:
Apparent mRNA Abundance for Hyaluronan Synthase1,2 and 3 genes in WT and HasKO tendons at 4, 8, and 12 weeks of age
| Gene | Age | WT1 | Has1KO1 | Has3KO1 | Has1/3KO2 | |||
|---|---|---|---|---|---|---|---|---|
| (weeks) | Abundance3 | Abundance3 | Fold4 | Abundance3 | Fold4 | Abundance3 | Fold4 | |
| Has1 | 4 | 3.35 (±1.43) | ND | - | 3.10 (±3.25) | −1.04 (±2.80) | ND | - |
| 8 | 2.98 | ND | - | 1.58 | −1.89 | ND | - | |
| 12 | 3.02 (±0.97) | ND | - | 3.95 (±2.41) | 1.36 (±0.83) | ND | - | |
| Has2 | 4 | 0.42 (±0.15) | 0.45 (±0.40) | −0.60 (±2.51) | 0.16 (±0.08) | −3.21 (±2.08) | 0.09 | −4.78 |
| 8 | 0.16 | 0.08 | −1.97 | 0.11 | −1.46 | 0.09 | −1.71 | |
| 12 | 0.29 (±0.18) | 0.45 (±0.54) | −0.13 (±3.95) | 0.26 (±0.13) | 0.12 (±2.09) | 0.22 | −1.13 | |
| Has3 | 4 | ND | ND | - | ND | - | ND | - |
| 8 | ND | ND | - | ND | - | ND | - | |
| 12 | ND | ND | - | ND | - | ND | - | |
Assays were performed on n=3 individual pools of tendons for 4 and 12 week time points, with average values ± standard deviations presented. For the 8 week time point only one pool was assayed. See Table 1 for details.
Assays were performed on one pool of tendons for all three time points. See Table 1 for details
Data were calculated as apparent mRNA abundance relative to Gapdh. See Methods for details.
Fold change in Has-deficient genotypes relative to WT. See Methods for details
Statistical evaluation showed no significant differences in apparent mRNA abundance for age-matched genotype comparisons and genotype-matched age comparisons (p>0.05).
ND=Not detectable (Ct values >34 cycles)