Table 3:
Apparent mRNA abundance of extracellular matrix genes in WT and HasKO tendons at 4, 8, and 12 weeks of age
| Gene | Age | WT1 | Has1KO1 | Has3KO1 | Has1/3KO2 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| (weeks) | Abundance3 | Sig5,6 | Abundance3 | Fold4 | Sig5,6 | Abundance3 | Fol4 | Sig5,6 | Abundance3 | Fold4 | |
| Collal | 4 | 36004 (±16423) | AAA | 10785(±4484) | −3.69 (±2.01) | E | 14637(±13743) | −3.77 (±2.37) | 11705 | −2.89 | |
| 8 | 2383 | 1417 | −1.68 | 9363 | 3.93 | 3688 | 1.55 | ||||
| 12 | 3699 (±958) | AAA | 2346 (±654.2) | −1.63 (±0.48) | E | 4206 (±1898) | −0.19 (±1.70) | 2591 | −1.39 | ||
| Col3al | 4 | 1248(±930) | B | 501.5 (±421.3) | −3.21 (±2.27) | FF | 763.5 (±572.1) | −1.11 (±2.17) | H | 202.8 | −5.05 |
| 8 | 90.43 | 33.73 | −2.68 | 133.5 | 1.48 | 58.8 | −1.54 | ||||
| 12 | 173.8 (±24.59) | B | 18.27 (±11.12) | −13.54 (±10.54) | FF *** | 152.1 (±79.03) | −0.630 (±1.77) | H | 193.4 | 1.12 | |
| Dcn | 4 | 5445(±2481) | 1637 (±391.0) | −3.23 (±0.69) | GG* | 5576 (±2780) | −0.29 (±1.74) | 2371.8 | −2.15 | ||
| 8 | 2864 | 2459 | −1.16 | 4763 | 1.66 | 2207 | −1.3 | ||||
| 12 | 7927 (±2521) | 133209(±9664) | 1.06 (±2.16) | GG | 4788 (±344.2) | −1.61 (±0.11) | 3456 | −2.22 | |||
| Bgn | 4 | 1565 (±220.4) | CCC | 604.9 (±123.2) | −2.65 (±0.57) | *** | 445.0 (±131.5) | −3.68 (±0.98) | *** | 438.9 | −3.54 |
| 8 | 183.3 | 177.5 | −1.07 | 246.2 | 1.3 | 164.9 | −1.15 | ||||
| 12 | 343.0 (±88.16) | CCC | 545.1 (±256.1) | 0.94 (±1.93) | 333.4 (±103.7) | 0.26 (±1.57) | 377.1 | 1.13 | |||
| Fmod | 4 | 4314 (±2319) | DD | 880.5 (±131.6) | −4.55 (±0.70) | ** | 2096(±1014) | −2.18 (±0.95) | 1268 | −3.11 | |
| 8 | 573.2 | 399.4 | −1.43 | 1087 | 1.9 | 671.3 | 1.17 | ||||
| 12 | 666.5 (±247.4) | DD | 913.3 (±187.5) | 1.43 (±0.29) | 1138 (±184.3) | 1.78 (±0.29) | 772.2 | 1.21 | |||
Assays were performed on n= 3 individual pools of tendons for 4 and 12 week time points, with average values ± standard deviations presented. For the 8 week time point only one pool was assayed. See Table 1 for details.
Assays were performed on one pool of tendons for all three time points. See Table 1 for details
Data were calculated as apparent mRNA abundance relative to Gapdh. See Methods for details.
Fold change in Has-deficient genotypes relative to WT. See Methods for details
Asterisks denote significant differences in expression between Has-deficient genotypes relative to age-matched WT (statistics performed on 4 and 12 week, Has1KO and Has3KO groups only;
p<0.05,
p<0.01,
p<0.005).
Matching letters (A-F) denote significant differences in expression between genotype-matched 4 and 12 week olds (example: A; p<0.05, AA; p<0.01, AAA; p<0.005).