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. 2017 Apr 24;15(9):858–867. doi: 10.1038/cmi.2017.15

Figure 2.

Figure 2

Knockdown of IFITM3 potentiates virus-triggered induction of IFN-β and inhibits viral replication. (a) HeLa cells were transfected with the control or indicated RNAi constructs. Cell lysates were analyzed on immunoblots with the indicated antibodies. Graphs show the means±s.d., n=3, *P<0.05, **P<0.01. (b and c) HEK293 cells were transfected with the indicated RNAi constructs together with the indicated reporter plasmids. The cells were then infected with SeV (b) or IFN-γ (c) for 12 h before luciferase assays were performed. (d–g) HEK293 cells were transfected with the indicated RNAi constructs. Cells were then infected with SeV (d and f) for 10 h or transfected with poly(I:C) (e and g) before real-time PCR (d and e) or ELISA (f and g) was performed. (h) HEK293 cells were transfected with control or IFITM3-RNAi construct for 36 h and then transfected with poly(I:C) for 24 h. The supernatants were collected and applied to Vero cells for 24 h. These Vero cells were infected with GFP-VSV or GFP-NDV (MOI=0.1) for 24 h and visualized by microscopy or analyzed on immunoblots with the indicated antibodies.