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. 2018 Oct 26;8:15895. doi: 10.1038/s41598-018-34256-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Venn diagram for the up- and down-regulated genes. (a) Venn diagram of the hp dag1/WT and ws dag1/WT up- and down-regulated DE genes. (b) Expression analysis of the M17, CRA1, EM6, LEA18, M10, At2g23110, At3g17520, and At3g53040 DE genes in dag1 and wild type hypocotyls under continuous monochromatic red light (40 μmolm⁻²s⁻¹). RT-qPCR assays were performed with 1 μl of the diluted cDNA, along with the specific primers, listed in Supplementary Table S3. Relative expression levels were normalized with UBQ10 (At4g05320) reference gene. The values of relative expression levels are means of three biological replicates, presented with SD values. Significant differences were analyzed by t-test (*P ≤ 0,05).