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. 2018 Oct 26;8:15890. doi: 10.1038/s41598-018-33565-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of different immobilization methods, BSA and glutaraldehyde (Method A), Chitosan and PVC (Method B), Chitosan alone (Method C) and Chitosan and glutaraldehyde (Method D). (A) Effect of Immobilization Method on Enzyme Binding Kinetics. 15.4 U/mL LOx was immobilized to Kapton films using four unique methods. Error bars indicate standard error of the mean. Note that x-axis values have been offset slightly for better visualization of error bars. The error bars for Method D are smaller than the markers. Trend lines represent least squares fit to the Michaelis-Menten equation. Enzyme kinetic data resulting from Method A resulted in the best fit to the model of the methods tested (R2 = 0.9551). (B) Stability of immobilization Method A demonstrated with a sample that has been tested, rinsed, and dried multiple times, and stored for more than 6 months in phosphate buffer. The material is still clearly adhered to the substrate.