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. 2018 Oct 26;9(11):1095. doi: 10.1038/s41419-018-1079-7

Fig. 2. Adult Mkk4- and Mkk7-deficient animals have fewer retinal ganglion cells resulting from postnatal dropout.

Fig. 2

a The number of TUJ-1-positive RGCs is significantly reduced in adult Mkk4- and Mkk7-deficient retinal flat mounts. Representative flat mounts are displayed to the right of the graph. (*P ≤ 0.05). N ≥ 10 retinas per genotype. b P0 flat mounts stained with BRN3B reveal normal RGC densities in both Mkk4- and Mkk7-deficient retinas, implying RGC dropout occurs postnatally. Representative flat mounts shown to the right of the graph. N ≥ 4 retinas per genotype. All examined Mkk7-deficient adult retinas displayed areas of abnormal fasciculation in both TUJ-1-positive RGCs (c; N ≥ 12) and tyrosine hydroxylase (TH)-positive amacrine cells (d; N ≥ 4). Scale bars: (a–c) 50 μm; (d) 100 μm. Error bars represent SEM