Figure 4.

The CD4⁺ T cell epitope-based heterologous prime-boost vaccination boosted the humoral responses in chronically infected mice. On day 8 post boost: (a) The number of virus-specific T_FH cells in the spleens of the control and vaccinated mice (n=5). (b) The frequency and number of GC B cells in the spleens of the control and vaccinated mice (n=4). (c) Titration of LCMV-specific IgG in the sera of the control and vaccinated mice (n=4). The data are representative of three independent experiments and were analyzed using two-tailed unpaired t-tests. The error bars denote the s.e.m. *P<0.05; ***P<0.001.