Fig. 4.

Mutant p53 increases ΔΨm without damaging mtDNA. a Melanoma cell lines A375 (WTp53) and MeWo (mutant p53) were transfected with control or siRNA-p53. H₂O₂ production was evaluated with the Amplex Red Kit, whereas UCP2 and p53 levels were determined by RT-qPCR. *p < 0.05. b Protein expression analysis of p53 and UCP2 was performed by western blotting and normalized to GAPDH expression. *p < 0.05. c Mitochondrial membrane potential (ΔΨm) was determined by TMRM fluorescencent probe, and mtDNA damage was evaluated with a qPCR approach as described in Material and Methods section. *p < 0.05 CTRL vs siRNA-p53. The experiments are representative of three biological replicates