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. 2018 Oct 26;9:4483. doi: 10.1038/s41467-018-06804-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Dual agonist/antagonist (site-1) anti-IL-7Rα mAbs induce transcriptional modification and activation of human leukocytes. a RNA-Seq analysis of human PBMCs (n = 7) incubated without IL-7 for 3.5 h with different anti-human IL-7Rα mAbs (10 µg/ml, blue: site-1/2b IgG4, red: site-1 IgG4#1, green: site-1 IgG1#2). Upper left: Venn diagram of the 481 differentially expressed genes (FDR 5%, FC > 1.5) comparing anti-IL-7Rα mAbs and medium control conditions. Upper right: Principal component analysis (PCA) of anti-IL-7Rα mAbs versus control and IL-7 stimulation (5 ng/ml) on the 93 most differentially expressed genes (FDR 5%, FC > 2) comparing IL-7 stimulation and control conditions. Bottom: Relative expression (fold-change as compare to control medium condition) of selected genes with each anti-IL-7Rα mAb (blue: site-1/2b IgG4, red: site-1 IgG4#1, green: site-1 IgG1#2). *p < 0.05; **p < 0.01; ***p < 0.001 as compare to control medium condition. b RNA-Seq analysis of human PBMCs (n = 7) incubated with IL-7 (5 ng/ml) for 3.5 h with the different anti-human IL-7Rα mAbs (10 µg/ml). Upper: Heatmap of the expression of the 93 most differentially expressed genes (FDR 5%, FC > 2) between IL-7 stimulation and control condition. Bottom: Quantification of the median profile of the three IL-7 induced clusters in IL-7 stimulated, control and IL-7 + anti-human IL-7Rα mAbs conditions. Same colors as in (a). *p < 0.05; **p < 0.01; ***p < 0.001 between indicated groups