Fig. 8.

Antagonist anti-IL-7Rα mAb inhibits antigen-specific human memory T cells persistence after antigen rechallenge. Human CPD-labeled PBMCs (n = 4) were stimulated with MHCI or MHCII -restricted pool of peptides (H1N1 flu; CEFT: CMV, EBV, Flu, Tetanos; CEF: CMV, EBV, Flu) or with anti-CD3 + anti-CD28 mAbs for 3 days (a), 8 days (b) or 10 days (c). Cells were cultured in medium alone (white histogram) or supplemented with 5 ng/ml of recombinant human IL-7 (black histogram) or 5 ng/mL of human IL-7 plus 10 µg/ml of the site-1/2b humanized anti-IL-7Rα mAb (grey histogram). The histograms show the percentage of proliferated cells (% of CPD^low cells) and viable cells (% of Annexin-V⁻ PI⁻ cells) among total cells, or within proliferated (CPDlow, Fig. 7c middle) versus quiescent (CPD high, Fig. 7c right) cells. Horizontal bars mean ± SEM. *p < 0.05 between indicated groups