Table 1.

Single-molecule measurements of membrane-dissociation kinetics and diffusion coefficients

	DdWT/ + LY	DdWT/ − LY	DdGE/ + LY	DdGE/ − LY	HsWT/ + LY	HsWT/ − LY
Dissociation
k1 [sec−1]	0.11 (n.d.)	0.22 (0.10)	0.13 (0.01)	0.18 (0.05)	0.13 (0.00)	0.32 (0.20)
k2 [sec−1]	1.04 (0.92)	1.37 (1.25)	1.05 (0.93)	1.36 (1.23)	0.63 (0.51)	1.85 (1.73)
k3 [sec−1]	4.82 (4.69)	5.01 (4.88)	5.06 (4.93)	4.14 (4.01)	4.79 (4.66)	6.30 (6.17)
q k 1	0.04	0.03	0.04	0.04	0.05	0.05
q k 2	0.28	0.22	0.25	0.27	0.17	0.25
q k 3	0.68	0.75	0.71	0.69	0.79	0.70
Residual	0.0060	0.0078	0.0036	0.0070	0.0066	0.0054
Lifetime [sec]	4.62	1.72	3.49	2.29	4.25	1.43
Diffusion
D1 [μm2 sec−1]	0.01	0.01	0.01	0.03	0.02	0.02
D2 [μm2 sec−1]	0.05	0.05	0.06	0.08	0.07	0.14
D3 [μm2 sec−1]	0.52	0.43	0.75	0.66	0.53	0.77
q D 1	0.67	0.41	0.60	0.44	0.66	0.40
q D 2	0.30	0.56	0.35	0.50	0.26	0.41
q D 3	0.03	0.03	0.05	0.06	0.08	0.19
Data number
Molecules	1322	1280	1318	1490	781	972
Cells	20	13	19	19	24	16

The parameters k_1/2/3 and q^k_1/2/3 were estimated by fitting the dissociation curve to equation (1) with the least-squares method. Squared 2-norms of the residuals are indicated. The parameters D_1/2/3 and q^D_1/2/3 were estimated by fitting the displacement distribution to equation (3) with the maximum likelihood estimation method. Numbers in parenthesis represents the rate constants after subtraction of the photo-bleaching rate constant